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Assistant Scientist I or II

Company: Aptevo Therapeutics
Location: Seattle
Posted on: April 17, 2018

Job Description:

I: Job Summary
The Assistant Scientist in the Molecular Biology and Protein Engineering group will play a critical role in the development and optimization of protein reagents and drug candidates based on the ADAPTIR platform, including monospecific and bispecific antibodies. Primary activities include plasmid vector design and cloning, DNA sequencing analysis, and phage display, with opportunities to grow into more advanced molecular engineering projects.
II: Responsibilites
Utilize standard molecular biology techniques, including bacterial cloning, DNA production and purification, site directed mutagenesis, Gibson Assembly, primer design, PCR and agarose gel electrophoresis to produce expression vectors encoding protein reagents and protein therapeutics
Participate in phage display panning to isolate new or optimized binding domains to targets of interest
Recombinant protein expression and characterization in in vitro assays
Cell culture and aseptic technique
Database entry and maintenance
Documentation of experiments and results in laboratory notebooks
Provide lab support while working in a collaborative team environment
III: Education, Experience & Skills
4 year college degree in Biology, Biochemistry or a related life sciences field
1 - 3 years of relevant academic or industrial experience in molecular biology or protein engineering is required.
Demonstrated competency in standard molecular biology techniques.
Experience with DNA software tools for plasmid and PCR sequence analysis (e.g. Vector NTI)
Outstanding organizational and record keeping skills, with a demonstrated ability to multi-task in a fast-paced environment.
Ability to meticulously maintain a lab notebook.
Excellent communication skills, independently motivated, detail oriented, and demonstrate good problem solving ability.
Familiarity with phage display a plus
IV: Physical/Mental Requirements
Type/keyboard, visual acuity, good eye/hand coordination, stand, walk, sit, twist/turn, climb, reach outward, reach above shoulder, hand dexterity, bend, squat/kneel, sense of feel, sense of hearing, sense of smell, lift/carry 25 lbs., and pull 25 lbs. Use phone, fax, copier, and computer. Use hand tools and operate fork lift. Organize/coordinate, analyze/interpret, calibrate precise measurements, problem solve, make decisions, supervise, plan, communicate, prepare written communications, and prepare transaction documents. Drive automatic/standard vehicle. Work outdoors, around noise above conversation level, in hot/cold temperatures, in high humidity, where ventilation is needed, in multiple locations, with restricted access to laboratory, in contact with Select Agents, and with exposure to animals, animal areas, or equipment used in animal areas.

Keywords: Aptevo Therapeutics, Seattle, Assistant Scientist I or II, Other, Seattle, Washington

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